Coding

Part:BBa_K2108000:Design

Designed by: Maya de Vos   Group: iGEM16_NorthernBC   (2016-10-13)


YiaT-MCS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that the multiple clonign site did nto contain any restriction sites that were also contained in the suffix and prefix.


Source

The sequence for the truncated Yiat protein was derived from: Han, M.J., Lee, S.H. (2014). An efficient bacterial surface display system based on a novel outer membrane anchoring element from the Escherichia coli protein YiaT. FEMS Microbiology Letters, 362, 1-7.

References